Brightness Characterization of Internal Endomembrane Proteins by Z-Scan Fluorescence Fluctuation Spectroscopy
نویسندگان
چکیده
منابع مشابه
Brightness analysis by Z-scan fluorescence fluctuation spectroscopy for the study of protein interactions within living cells.
Fluorescence fluctuation spectroscopy (FFS) quantifies interactions of fluorescently labeled proteins inside living cells by brightness analysis. Conventional FFS implicitly requires that the sample thickness exceeds the size of the observation volume. This condition is not always fulfilled when measuring cells. Cytoplasmic sections, especially, can be thinner than the axial size of the observa...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2013.11.1169